Congratulations to Ignacio and Dr. Burgess for publishing the first synthetic biology paper in the lab – a brief communication on a Golden Gate toolkit for continuous directed evolution in E. coli (but compatible with other photoautotrophic organisms 😉- stay tuned…).
In this paper, we show that complex mutator plasmids can be assembled in under 10 days in a workflow compatible with the modular cloning system most labs are using these days (Plant MoClo). We also show that SP6 RNA polymerase is a viable substitute for T7 RNA polymerase for the purpose of inducing mutations – potentially freeing up T7 for other purposes in whatever gene circuit researchers are working with. Pretty cool!
Interested? Read the article here: https://doi.org/10.1093/synbio/ysaf014
Burgess Lab 